Development, validation and use of ELISA for antibodies to human alpha-1 antitrypsin
G Ye, RA Oshins, FN Rouhani, ML Brantly… - Journal of immunological …, 2013 - Elsevier
Evaluation of human antibody responses to alpha-1 antitrypsin (AAT) in clinical trials and
clinical practice has been limited by the lack of a validated assay. Here we describe the
development and validation of an ELISA method for quantification of human and nonhuman
primate antibody responses to human AAT. A reference anti-human AAT serum standard
was generated using sera from a cynomolgus macaque injected with a recombinant adeno-
associated virus vector expressing human AAT. The ELISA was validated for use with …
clinical practice has been limited by the lack of a validated assay. Here we describe the
development and validation of an ELISA method for quantification of human and nonhuman
primate antibody responses to human AAT. A reference anti-human AAT serum standard
was generated using sera from a cynomolgus macaque injected with a recombinant adeno-
associated virus vector expressing human AAT. The ELISA was validated for use with …