A simple hanging drop cell culture protocol for generation of 3D spheroids
R Foty - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Studies of cell-cell cohesion and cell-substratum adhesion have historically been performed
on monolayer cultures adherent to rigid substrates. Cells within a tissue, however, are
typically encased within a closely packed tissue mass in which cells establish intimate
connections with many near-neighbors and with extracellular matrix components.
Accordingly, the chemical milieu and physical forces experienced by cells within a 3D tissue
are fundamentally different than those experienced by cells grown in monolayer culture. This …
on monolayer cultures adherent to rigid substrates. Cells within a tissue, however, are
typically encased within a closely packed tissue mass in which cells establish intimate
connections with many near-neighbors and with extracellular matrix components.
Accordingly, the chemical milieu and physical forces experienced by cells within a 3D tissue
are fundamentally different than those experienced by cells grown in monolayer culture. This …