Detection of protein S-nitrosylation with the biotin-switch technique

MT Forrester, MW Foster, M Benhar… - Free Radical Biology and …, 2009 - Elsevier
Free Radical Biology and Medicine, 2009Elsevier
Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-
nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have
demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and
recent efforts have greatly advanced our scientific understanding of how this redox-based
modification is dynamically regulated during physiological and pathophysiological
conditions. The focus of this review is the biotin-switch technique (BST), which has become …
Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and recent efforts have greatly advanced our scientific understanding of how this redox-based modification is dynamically regulated during physiological and pathophysiological conditions. The focus of this review is the biotin-switch technique (BST), which has become a mainstay assay for detecting S-nitrosylated proteins in complex biological systems. Potential pitfalls and modern adaptations of the BST are discussed, as are future directions for this assay in the burgeoning field of protein S-nitrosylation.
Elsevier