[HTML][HTML] Mapping of the discontinuous H-kininogen binding site of plasma prekallikrein: evidence for a critical role of apple domain-2
T Renné, J Dedio, W Müller-Esterl, JCM Meijers… - Journal of Biological …, 1999 - ASBMB
Plasma prekallikrein, a zymogen of the contact phase system, circulates in plasma as
heterodimeric complex with H-kininogen. The binding is mediated by the prekallikrein heavy
chain consisting of four apple domains, A1 to A4, to which H-kininogen binds with high
specificity and affinity (KD= 1.2× 10− 8 m). Previous work had demonstrated that a
discontinuous kininogen-binding site is formed by a proximal part located in A1, a distal part
exposed by A4, and other yet unidentified portion (s) of the kallikrein heavy chain. To detect …
heterodimeric complex with H-kininogen. The binding is mediated by the prekallikrein heavy
chain consisting of four apple domains, A1 to A4, to which H-kininogen binds with high
specificity and affinity (KD= 1.2× 10− 8 m). Previous work had demonstrated that a
discontinuous kininogen-binding site is formed by a proximal part located in A1, a distal part
exposed by A4, and other yet unidentified portion (s) of the kallikrein heavy chain. To detect …