Demonstration of the chemotactic properties of collagen

C Chang, JC Houck - … of the Society for Experimental Biology …, 1970 - journals.sagepub.com
C Chang, JC Houck
Proceedings of the Society for Experimental Biology and Medicine, 1970journals.sagepub.com
An in vivo method for measuring chemotaxis has been described using Millipore filters. This
method demonstrated that soluble collagen, but not gelatin, was quite chemotactic. Further,
the products of collagenolysis produced by cutaneous collagenase, but not by bacterial
collagenase, were extraordinarily chemotactic for polymorphonuclear leukocytes within 4 hr
after testing at a concentration of about 0.1 μg.
Summary
An in vivo method for measuring chemotaxis has been described using Millipore filters. This method demonstrated that soluble collagen, but not gelatin, was quite chemotactic. Further, the products of collagenolysis produced by cutaneous collagenase, but not by bacterial collagenase, were extraordinarily chemotactic for polymorphonuclear leukocytes within 4 hr after testing at a concentration of about 0.1 μg.
Sage Journals