[HTML][HTML] Seventeen-colour flow cytometry: unravelling the immune system

SP Perfetto, PK Chattopadhyay… - Nature Reviews …, 2004 - nature.com
Nature Reviews Immunology, 2004nature.com
The increasing need for polychromatic approaches to flow cytometry, coupled with rapid
technological advances, has pushed the frontiers of flow cytometry beyond 12-colour
systems. Recent breakthroughs have allowed the design and implementation of instruments
that measure 19 parameters (17 fluorescent colours and 2 physical parameters). This article
describes the instrumentation and considers the reagents, analysis and applications for this
powerful, new extension of flow-cytometric technology.
Abstract
The increasing need for polychromatic approaches to flow cytometry, coupled with rapid technological advances, has pushed the frontiers of flow cytometry beyond 12-colour systems. Recent breakthroughs have allowed the design and implementation of instruments that measure 19 parameters (17 fluorescent colours and 2 physical parameters). This article describes the instrumentation and considers the reagents, analysis and applications for this powerful, new extension of flow-cytometric technology.
nature.com