Calpain-mediated cleavage negatively regulates the expression level of ABCG1
N Hori, H Hayashi, Y Sugiyama - Atherosclerosis, 2011 - Elsevier
OBJECTIVE: The ATP-binding cassette transporter ABCG1 mediates cholesterol efflux from
macrophages, and prevents the progression of macrophage foam-cell formation. Much less
is known about the regulatory mechanism of ABCG1, although its physiological importance
is becoming clearer. Here, we show the role of calpain in ABCG1 degradation. METHODS
AND RESULTS: Purified μ-calpain cleaved ABCG1 in crude membrane fractions prepared
from human ABCG1-expressing HEK293 (ABCG1-HEK) cells. In ABCG1-HEK cells …
macrophages, and prevents the progression of macrophage foam-cell formation. Much less
is known about the regulatory mechanism of ABCG1, although its physiological importance
is becoming clearer. Here, we show the role of calpain in ABCG1 degradation. METHODS
AND RESULTS: Purified μ-calpain cleaved ABCG1 in crude membrane fractions prepared
from human ABCG1-expressing HEK293 (ABCG1-HEK) cells. In ABCG1-HEK cells …