[HTML][HTML] The urea decomposition product cyanate promotes endothelial dysfunction

D El-Gamal, SP Rao, M Holzer, S Hallström… - Kidney international, 2014 - Elsevier
D El-Gamal, SP Rao, M Holzer, S Hallström, J Haybaeck, M Gauster, C Wadsack, A Kozina…
Kidney international, 2014Elsevier
The dramatic cardiovascular mortality of patients with chronic kidney disease is attributable
in a significant proportion to endothelial dysfunction. Cyanate, a reactive species in
equilibrium with urea, is formed in excess in chronic kidney disease. Cyanate is thought to
have a causal role in promoting cardiovascular disease, but the underlying mechanisms
remain unclear. Immunohistochemical analysis performed in the present study revealed that
carbamylated epitopes associate mainly with endothelial cells in human atherosclerotic …
The dramatic cardiovascular mortality of patients with chronic kidney disease is attributable in a significant proportion to endothelial dysfunction. Cyanate, a reactive species in equilibrium with urea, is formed in excess in chronic kidney disease. Cyanate is thought to have a causal role in promoting cardiovascular disease, but the underlying mechanisms remain unclear. Immunohistochemical analysis performed in the present study revealed that carbamylated epitopes associate mainly with endothelial cells in human atherosclerotic lesions. Cyanate treatment of human coronary artery endothelial cells reduced expression of endothelial nitric oxide synthase, and increased tissue factor and plasminogen activator inhibitor-1 expression. In mice, administration of cyanate, promoting protein carbamylation at levels observed in uremic patients, attenuated arterial vasorelaxation of aortic rings in response to acetylcholine without affecting the sodium nitroprusside–induced relaxation. Total endothelial nitric oxide synthase and nitric oxide production were significantly reduced in aortic tissue of cyanate-treated mice. This coincided with a marked increase of tissue factor and plasminogen activator inhibitor-1 protein levels in aortas of cyanate-treated mice. Thus, cyanate compromises endothelial functionality in vitro and in vivo. This may contribute to the dramatic cardiovascular risk of patients suffering from chronic kidney disease.
Elsevier