[HTML][HTML] Identification of Tek/Tie2 binding partners: binding to a multifunctional docking site mediates cell survival and migration

N Jones, Z Master, J Jones, D Bouchard, Y Gunji… - Journal of Biological …, 1999 - ASBMB
The Tek/Tie2 receptor tyrosine kinase plays a pivotal role in vascular and hematopoietic
development. To study the signal transduction pathways that are mediated by this receptor,
we have used the yeast two-hybrid system to identify signaling molecules that associate with
the phosphorylated Tek receptor. Using this approach, we demonstrate that five molecules,
Grb2, Grb7, Grb14, Shp2, and the p85 subunit of phosphatidylinositol 3-kinase can interact
with Tek in a phosphotyrosine-dependent manner through their SH2 domains. Mapping of …