[HTML][HTML] Suppression of Tie-1 in endothelial cells in vitro induces a change in the genome-wide expression profile reflecting an inflammatory function

B Chan, VP Sukhatme - FEBS letters, 2009 - Elsevier
B Chan, VP Sukhatme
FEBS letters, 2009Elsevier
Tie-1 is an endothelial specific receptor tyrosine kinase that is upregulated in diseases such
as atherosclerosis and rheumatoid arthritis. We recently demonstrated that Tie-1 induced a
proinflammatory response when overexpressed in endothelial cells. Here, we used a
complementary approach and suppressed endogenous Tie-1 expression in endothelial
cells to examine its function by microarray analysis. Tie-1 appeared to govern expression of
many genes involved in inflammation. Expression knockdown of Tie-1 significantly reduced …
Tie-1 is an endothelial specific receptor tyrosine kinase that is upregulated in diseases such as atherosclerosis and rheumatoid arthritis. We recently demonstrated that Tie-1 induced a proinflammatory response when overexpressed in endothelial cells. Here, we used a complementary approach and suppressed endogenous Tie-1 expression in endothelial cells to examine its function by microarray analysis. Tie-1 appeared to govern expression of many genes involved in inflammation. Expression knockdown of Tie-1 significantly reduced endothelial conditioned medium ability to stimulate MCP-1 production in U937 cells. Collectively, our results support the notion that Tie-1 has an inflammatory function in endothelial cells.
Elsevier