[HTML][HTML] Effector memory T cells, early metastasis, and survival in colorectal cancer

F Pagès, A Berger, M Camus… - New England journal …, 2005 - Mass Medical Soc
F Pagès, A Berger, M Camus, F Sanchez-Cabo, A Costes, R Molidor, B Mlecnik, A Kirilovsky
New England journal of medicine, 2005Mass Medical Soc
Background The role of tumor-infiltrating immune cells in the early metastatic invasion of
colorectal cancer is unknown. Methods We studied pathological signs of early metastatic
invasion (venous emboli and lymphatic and perineural invasion) in 959 specimens of
resected colorectal cancer. The local immune response within the tumor was studied by flow
cytometry (39 tumors), low-density-array real-time polymerase-chain-reaction assay (75
tumors), and tissue microarrays (415 tumors). Results Univariate analysis showed significant …
Background
The role of tumor-infiltrating immune cells in the early metastatic invasion of colorectal cancer is unknown.
Methods
We studied pathological signs of early metastatic invasion (venous emboli and lymphatic and perineural invasion) in 959 specimens of resected colorectal cancer. The local immune response within the tumor was studied by flow cytometry (39 tumors), low-density-array real-time polymerase-chain-reaction assay (75 tumors), and tissue microarrays (415 tumors).
Results
Univariate analysis showed significant differences in disease-free and overall survival according to the presence or absence of histologic signs of early metastatic invasion (P<0.001). Multivariate Cox analysis showed that an early conventional pathological tumor–node–metastasis stage (P<0.001) and the absence of early metastatic invasion (P=0.04) were independently associated with increased survival. As compared with tumors with signs of early metastatic invasion, tumors without such signs had increased infiltrates of immune cells and increased levels of messenger RNA (mRNA) for products of type 1 helper effector T cells (CD8, T-BET [T-box transcription factor 21], interferon regulatory factor 1, interferon-γ, granulysin, and granzyme B) but not increased levels of inflammatory mediators or immunosuppressive molecules. The two types of tumors had significant differences in the levels of expression of 65 combinations of T-cell markers, and hierarchical clustering showed that markers of T-cell migration, activation, and differentiation were increased in tumors without signs of early metastatic invasion. The latter type of tumors also had increased numbers of CD8+ T cells, ranging from early memory (CD45RO+CCR7–CD28+CD27+) to effector memory (CD45RO+CCR7–CD28–CD27–) T cells. The presence of high levels of infiltrating memory CD45RO+ cells, evaluated immunohistochemically, correlated with the absence of signs of early metastatic invasion, a less advanced pathological stage, and increased survival.
Conclusions
Signs of an immune response within colorectal cancers are associated with the absence of pathological evidence of early metastatic invasion and with prolonged survival.
The New England Journal Of Medicine