Up-regulation of hypoxia inducible factor 1α by isoflurane in Hep3B cells
QF Li, XR Wang, YW Yang, DS Su - The Journal of the American …, 2006 - pubs.asahq.org
Background The volatile anesthetic isoflurane induces hypoxia inducible factor (HIF)-1-
responsive genes heme oxygenase 1, inducible nitric oxide synthase, and vascular
endothelial growth factor (VEGF) expression. Little is known about the extent to which
induction of HIF-1alpha is affected by isoflurane. Methods Hep3B cells were exposed to
isoflurane at various concentrations (0.5-4%) or for different time periods (2-8 h) at 37
degrees C. HIF-1alpha gene expression and transcriptional activity, heme oxygenase 1 …
responsive genes heme oxygenase 1, inducible nitric oxide synthase, and vascular
endothelial growth factor (VEGF) expression. Little is known about the extent to which
induction of HIF-1alpha is affected by isoflurane. Methods Hep3B cells were exposed to
isoflurane at various concentrations (0.5-4%) or for different time periods (2-8 h) at 37
degrees C. HIF-1alpha gene expression and transcriptional activity, heme oxygenase 1 …