Studies over the past four decades have established that Ca²⁺ is a critical factor in control of salivary gland function and have led to identification of the critical components of this process. The major ion transport mechanisms and ion channels that are involved in fluid secretion have also been established. The key event in activation of fluid secretion is an increase in [Ca²⁺]_i triggered by inositol 1,4,5‐trisphosphate (IP₃)‐induced release of Ca²⁺ from ER via the IP₃ receptor (IP₃R). IP₃Rs determine the site of initiation and the pattern of the [Ca²⁺]_i signal in the cell. However, Ca²⁺ entry into the cell is required to sustain the elevation of [Ca²⁺]_i and fluid secretion and is mediated by the store‐operated Ca²⁺ entry (SOCE) mechanism. Orai1, TRPC1, TRPC3 and STIM1 have been identified as critical components of SOCE in these cells. Cells finely tune the generation and amplification of [Ca²⁺]_i signals for regulation of cell function. An important emerging area is the concept that unregulated [Ca²⁺]_i signals in cells can directly cause cell damage, dysfunction and disease. Alternatively, aberrant [Ca²⁺]_i signals can also amplify and increase the rates of cell damage. Such defects in Ca²⁺ signalling have been described in salivary glands in conjunction with radiation‐induced loss of salivary gland function as well as in the salivary defects associated with the autoimmune exocrinopathy Sjögren's syndrome. Such defects have been associated with altered function or expression of key Ca²⁺ signalling components, such as STIM proteins and TRP channels. These studies offer new avenues for examining the mechanisms underlying the disease and development of novel clinical targets and therapeutic strategies.