Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity
J Degen, P Dublin, J Zhang, R Dobrowolski… - The FASEB …, 2012 - Wiley Online Library
The FASEB Journal, 2012•Wiley Online Library
Gene inactivation reporters are powerful tools to circumvent limitations of the widely used
Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines
expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43)
after Cre‐mediated recombination, we demonstrate dual reporter approaches to
simultaneously examine astrocyte subpopulations expressing different connexins, identify
compensatory up‐regulation within gene families, and quantify Cre‐mediated deletion at the …
Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines
expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43)
after Cre‐mediated recombination, we demonstrate dual reporter approaches to
simultaneously examine astrocyte subpopulations expressing different connexins, identify
compensatory up‐regulation within gene families, and quantify Cre‐mediated deletion at the …
Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre‐mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up‐regulation within gene families, and quantify Cre‐mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock‐in ECFP mouse revealed an unexpected heterogeneity of Cx43‐expressing astrocytes across brain areas.—Degen, J., Dublin, P., Zhang, J., Dobrowolski, R., Jokwitz, M., Karram, K., Trotter, J., Jabs, R., Willecke, K., Steinhäuser, C., Theis, M. Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity. FASEB J. 26, 4576–4583 (2012). www.fasebj.org
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