Galectin‐1 is essential for the induction of MOG35–55‐based intravenous tolerance in experimental autoimmune encephalomyelitis

ER Mari, J Rasouli, B Ciric, JN Moore… - European journal of …, 2016 - Wiley Online Library
ER Mari, J Rasouli, B Ciric, JN Moore, JR Conejo‐Garcia, N Rajasagi, GX Zhang…
European journal of immunology, 2016Wiley Online Library
In experimental autoimmune encephalomyelitis (EAE), intravenous (iv) injection of the
antigen, myelin oligodendrocyte glycoprotein‐derived peptide, MOG35–55, suppresses
disease development, a phenomenon called iv tolerance. Galectin‐1, an endogenous
glycan‐binding protein, is upregulated during autoimmune neuroinflammation and plays
immunoregulatory roles by inducing tolerogenic dendritic cells (DCs) and IL‐10 producing
regulatory type 1 T (Tr1) cells. To examine the role of galectin‐1 in iv tolerance, we …
In experimental autoimmune encephalomyelitis (EAE), intravenous (i.v.) injection of the antigen, myelin oligodendrocyte glycoprotein‐derived peptide, MOG35–55, suppresses disease development, a phenomenon called i.v. tolerance. Galectin‐1, an endogenous glycan‐binding protein, is upregulated during autoimmune neuroinflammation and plays immunoregulatory roles by inducing tolerogenic dendritic cells (DCs) and IL‐10 producing regulatory type 1 T (Tr1) cells. To examine the role of galectin‐1 in i.v. tolerance, we administered MOG35–55‐i.v. to wild‐type (WT) and galectin‐1 deficient (Lgals1−/−) mice with ongoing EAE. MOG35–55 suppressed disease in the WT, but not in the Lgals1−/− mice. The numbers of Tr1 cells and Treg cells were increased in the CNS and periphery of tolerized WT mice. In contrast, Lgals1−/− MOG‐i.v. mice had reduced numbers of Tr1 cells and Treg cells in the CNS and periphery, and reduced IL‐27, IL‐10, and TGF‐β1 expression in DCs in the periphery. DCs derived from i.v.‐tolerized WT mice suppressed disease when adoptively transferred into mice with ongoing EAE, whereas DCs from Lgals1−/− MOG‐i.v. mice were not suppressive. These findings demonstrate that galectin‐1 is required for i.v. tolerance induction, likely via induction of tolerogenic DCs leading to enhanced development of Tr1 cells, Treg cells, and downregulation of proinflammatory responses.
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