[HTML][HTML] A novel type of E3 ligase for the Ufm1 conjugation system
K Tatsumi, Y Sou, N Tada, E Nakamura… - Journal of biological …, 2010 - ASBMB
The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ubiquitin-like modifier
whose conjugation (ufmylation) system is conserved in multicellular organisms. Ufm1 is
known to covalently attach with cellular protein (s) via a specific E1-activating enzyme
(Uba5) and an E2-conjugating enzyme (Ufc1), but its E3-ligating enzyme (s) as well as the
target protein (s) remain unknown. Herein, we report both a novel E3 ligase for Ufm1,
designated Ufl1, and an Ufm1-specific substrate ligated by Ufl1, C20orf116. Ufm1 was …
whose conjugation (ufmylation) system is conserved in multicellular organisms. Ufm1 is
known to covalently attach with cellular protein (s) via a specific E1-activating enzyme
(Uba5) and an E2-conjugating enzyme (Ufc1), but its E3-ligating enzyme (s) as well as the
target protein (s) remain unknown. Herein, we report both a novel E3 ligase for Ufm1,
designated Ufl1, and an Ufm1-specific substrate ligated by Ufl1, C20orf116. Ufm1 was …