[HTML][HTML] Regulation of E2 promoter binding factor 1 (E2F1) transcriptional activity through a deubiquitinating enzyme, UCH37
CS Mahanic, V Budhavarapu, JD Graves, G Li… - Journal of Biological …, 2015 - ASBMB
E2F1 is tightly controlled by multiple mechanisms, but whether ubiquitination regulates its
transcriptional activity remains unknown. Here we identify UCH37 as the first, to our
knowledge, deubiquitinating enzyme for E2F1. UCH37 does not deubiquitinate UbK48
chains or affect E2F1 protein stability. Instead, UCH37, but not a catalytically dead mutant,
decreases the Lys-63-linked ubiquitination of E2F1 and activates its transcriptional activity.
UCH37 depletion reduces the gene expression of both proliferative and pro-apoptotic E2F1 …
transcriptional activity remains unknown. Here we identify UCH37 as the first, to our
knowledge, deubiquitinating enzyme for E2F1. UCH37 does not deubiquitinate UbK48
chains or affect E2F1 protein stability. Instead, UCH37, but not a catalytically dead mutant,
decreases the Lys-63-linked ubiquitination of E2F1 and activates its transcriptional activity.
UCH37 depletion reduces the gene expression of both proliferative and pro-apoptotic E2F1 …