Autoradiographic localisation of [3H] GABA and [3H] glutamate over satellite glial cells
F Schon, JS Kelly - Brain Research, 1974 - Elsevier
F Schon, JS Kelly
Brain Research, 1974•ElsevierThe uptake of the putative amino acids transmitter substances GABA, glycine and glutamate
was studied autoradiographically in adult rat sensory ganglia followingin vitro incubation. As
these ganglia are devoid of any synaptic innervation, they offer a convenient preparation for
examining glial cell uptake. GABA was exclusively localised within the satellite glial cells
surrounding unlabelled sensory neuronal cell bodies. GABA was also accumulated within
glial cells surrounding unmyelinated axons and in the Schwann cell bodies of the large …
was studied autoradiographically in adult rat sensory ganglia followingin vitro incubation. As
these ganglia are devoid of any synaptic innervation, they offer a convenient preparation for
examining glial cell uptake. GABA was exclusively localised within the satellite glial cells
surrounding unlabelled sensory neuronal cell bodies. GABA was also accumulated within
glial cells surrounding unmyelinated axons and in the Schwann cell bodies of the large …
Abstract
The uptake of the putative amino acids transmitter substances GABA, glycine and glutamate was studied autoradiographically in adult rat sensory ganglia followingin vitro incubation. As these ganglia are devoid of any synaptic innervation, they offer a convenient preparation for examining glial cell uptake.
GABA was exclusively localised within the satellite glial cells surrounding unlabelled sensory neuronal cell bodies. GABA was also accumulated within glial cells surrounding unmyelinated axons and in the Schwann cell bodies of the large myelinated fibres in the dorsal root ganglia.
Glutamate was also exclusively taken up into satellite glial cell bodies. The lack of accumulation in sensory neuronal cell bodies was surprising in view of the suggested role of glutamate as the possible mammalian sensory transmitter substance.
The uptake of glycine, leucine and alanine was much less than that for GABA or glutamate. All 3 amino acids were localised equally within neuronal and satellite glial cell bodies presumably by the neutral amino acid uptake system present in all cells.
Elsevier