[HTML][HTML] CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and …
PA Havre, LH Dang, K Ohnuma, S Iwata, C Morimoto… - BMC cancer, 2013 - Springer
PA Havre, LH Dang, K Ohnuma, S Iwata, C Morimoto, NH Dang
BMC cancer, 2013•SpringerBackground CD26/dipeptidyl peptidase IV (DPPIV) is a multifunctional membrane protein
with a key role in T-cell biology and also serves as a marker of aggressive cancers,
including T-cell malignancies. Methods Versican expression was measured by real-time RT-
PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was
performed using transduction-ready viral particles. The effect of versican depletion on
surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation …
with a key role in T-cell biology and also serves as a marker of aggressive cancers,
including T-cell malignancies. Methods Versican expression was measured by real-time RT-
PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was
performed using transduction-ready viral particles. The effect of versican depletion on
surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation …
Background
CD26/dipeptidyl peptidase IV (DPPIV) is a multifunctional membrane protein with a key role in T-cell biology and also serves as a marker of aggressive cancers, including T-cell malignancies.
Methods
Versican expression was measured by real-time RT-PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was performed using transduction-ready viral particles. The effect of versican depletion on surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation. CD44 secretion/cleavage and ERK (1/2) activation was followed by Western blotting. Collagenase I activity was measured by a live cell assay and in vesicles using a liquid-phase assay. Adhesion to collagen I was quantified by an MTS assay.
Results
Versican expression was down-regulated in CD26-depleted Karpas 299 cells compared to the parental T-ALCL Karpas 299 cells. Knock down of versican in the parental Karpas 299 cells led to decreased MT1-MMP surface expression as well as decreased CD44 expression and secretion of the cleaved form of CD44. Parental Karpas 299 cells also exhibited higher collagenase I activity and greater adhesion to collagenase I than CD26-knockdown or versican-knockdown cells. ERK activation was also highest in parental Karpas 299 cells compared to CD26-knockdown or versican-knockdown clones.
Conclusions
Our data indicate that CD26 has a key role in cell adhesion and invasion, and potentially in tumorigenesis of T-cell lines, through its association with molecules and signal transduction pathways integral to these processes.
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