[HTML][HTML] SINK is a p65-interacting negative regulator of NF-κB-dependent transcription

M Wu, LG Xu, Z Zhai, HB Shu - Journal of Biological Chemistry, 2003 - ASBMB
The transcription factor NF-κB plays important roles in inflammation and cell survival. In this
study, we identified SINK, an NF-κB-inducible protein. Overexpression of SINK inhibited NF-
κB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its
downstream signaling proteins but did not inhibit NF-κB translocation to the nucleus and
binding to DNA. Co-immunoprecipitation and in vitro kinase assays indicated that SINK
specifically interacted with the NF-κB transactivator p65 and inhibited p65 phosphorylation …