[HTML][HTML] Genome-wide analysis of transcription factor E2F1 mutant proteins reveals that N-and C-terminal protein interaction domains do not participate in targeting …
AR Cao, R Rabinovich, M Xu, X Xu, VX Jin… - Journal of Biological …, 2011 - ASBMB
Previous studies of E2F family members have suggested that protein-protein interactions
may be the mechanism by which E2F proteins are recruited to specific genomic regions. We
have addressed this hypothesis on a genome-wide scale using ChIP-seq analysis of MCF7
cell lines that express tagged wild type and mutant E2F1 proteins. First, we performed ChIP-
seq for tagged WT E2F1. Then, we analyzed E2F1 proteins that lacked the N-terminal SP1
and cyclin A binding domains, the C-terminal transactivation and pocket protein binding …
may be the mechanism by which E2F proteins are recruited to specific genomic regions. We
have addressed this hypothesis on a genome-wide scale using ChIP-seq analysis of MCF7
cell lines that express tagged wild type and mutant E2F1 proteins. First, we performed ChIP-
seq for tagged WT E2F1. Then, we analyzed E2F1 proteins that lacked the N-terminal SP1
and cyclin A binding domains, the C-terminal transactivation and pocket protein binding …