Reproducible mouse sciatic nerve crush and subsequent assessment of regeneration by whole mount muscle analysis
AR Bauder, TA Ferguson - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Regeneration in the peripheral nervous system (PNS) is widely studied both for its relevance
to human disease and to understand the robust regenerative response mounted by PNS
neurons thereby possibly illuminating the failures of CNS regeneration1. Sciatic nerve crush
(axonotmesis) is one of the most common models of peripheral nerve injury in rodents2.
Crushing interrupts all axons but Schwann cell basal laminae are preserved so that
regeneration is optimal3, 4. This allows the investigator to study precisely the ability of a …
to human disease and to understand the robust regenerative response mounted by PNS
neurons thereby possibly illuminating the failures of CNS regeneration1. Sciatic nerve crush
(axonotmesis) is one of the most common models of peripheral nerve injury in rodents2.
Crushing interrupts all axons but Schwann cell basal laminae are preserved so that
regeneration is optimal3, 4. This allows the investigator to study precisely the ability of a …