[HTML][HTML] Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: functional and therapeutic implications
D Corvetta, O Chayka, S Gherardi, CW D'Acunto… - Journal of Biological …, 2013 - ASBMB
CLU (clusterin) is a tumor suppressor gene that we have previously shown to be negatively
modulated by the MYCN proto-oncogene, but the mechanism of repression was unclear.
Here, we show that MYCN inhibits the expression of CLU by direct interaction with the non-
canonical E box sequence CACGCG in the 5′-flanking region. Binding of MYCN to the
CLU gene induces bivalent epigenetic marks and recruitment of repressive proteins such as
histone deacetylases and Polycomb members. MYCN physically binds in vitro and in vivo to …
modulated by the MYCN proto-oncogene, but the mechanism of repression was unclear.
Here, we show that MYCN inhibits the expression of CLU by direct interaction with the non-
canonical E box sequence CACGCG in the 5′-flanking region. Binding of MYCN to the
CLU gene induces bivalent epigenetic marks and recruitment of repressive proteins such as
histone deacetylases and Polycomb members. MYCN physically binds in vitro and in vivo to …