Differentiation-dependent expression of Adhfe1 in adipogenesis

JY Kim, KS Tillison, S Zhou, JH Lee… - Archives of biochemistry …, 2007 - Elsevier
JY Kim, KS Tillison, S Zhou, JH Lee, CM Smas
Archives of biochemistry and biophysics, 2007Elsevier
We have determined that adipocytes are a major site of expression of the transcript for the
novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its
encoded protein places it among the iron-activated ADHs. Western blot analysis reveals
Adhfe1 encodes a 50kDa protein and immunocytochemical staining indicates mitochondrial
localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro
brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 …
We have determined that adipocytes are a major site of expression of the transcript for the novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its encoded protein places it among the iron-activated ADHs. Western blot analysis reveals Adhfe1 encodes a 50kDa protein and immunocytochemical staining indicates mitochondrial localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 transcript expression in adipocytes is refractory to TNFα-mediated downregulation. However, use of pharmacological inhibitors reveals PI 3-kinase-mediated signals maintain the basal level of Adhfe1 transcript in 3T3-L1 adipocytes. Tissue profiling studies show Adhfe1 transcript is restricted to white and brown adipose tissues, liver, and kidney. In comparison to C57BL/6 mice, Adhfe1 transcript is downregulated 40% in white adipose tissue of ob/ob obese mice. Further characterization of Adhfe1 should yield new insights into adipocyte function and energy metabolism.
Elsevier