[HTML][HTML] Knockout of the α2 but not α1 5′-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-β-4-ribofuranosidebut not contraction …

SB Jørgensen, B Viollet, F Andreelli, C Frøsig… - Journal of Biological …, 2004 - ASBMB
We investigated the importance of the two catalytic α-isoforms of the 5′-AMP-activated
protein kinase (AMPK) in 5-aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR)
and contraction-induced glucose uptake in skeletal muscle. Incubated soleus and EDL
muscle from whole-body α 2-or α 1-AMPK knockout (KO) and wild type (WT) mice were
incubated with 2.0 mm AICAR or electrically stimulated to contraction. Both AICAR and
contraction increased 2DG uptake in WT muscles. KO of α 2, but not α 1, abolished AICAR …