TMPRSS2-ERG fusion heterogeneity in multifocal prostate cancer: clinical and biologic implications
OBJECTIVES: To characterize the clonality of TMPRSS2-ERG fusion in multifocal prostate
cancer. METHODS: From 80 consecutive radical prostatectomy specimens, we identified 32
cases with multiple spatially separate tumors. In each case, we assessed two to three tumor
foci for TMPRSS2-ERG fusion using an ERG break-apart interphase fluorescence in situ
hybridization assay. RESULTS: Individual tumor foci showed homogeneity for fusion status
(intrafocal clonal homogeneity). In 19 (59%) of the 32 cases, all foci within a case had the …
cancer. METHODS: From 80 consecutive radical prostatectomy specimens, we identified 32
cases with multiple spatially separate tumors. In each case, we assessed two to three tumor
foci for TMPRSS2-ERG fusion using an ERG break-apart interphase fluorescence in situ
hybridization assay. RESULTS: Individual tumor foci showed homogeneity for fusion status
(intrafocal clonal homogeneity). In 19 (59%) of the 32 cases, all foci within a case had the …