ATAC‐seq: a method for assaying chromatin accessibility genome‐wide

JD Buenrostro, B Wu, HY Chang… - Current protocols in …, 2015 - Wiley Online Library
Current protocols in molecular biology, 2015Wiley Online Library
This unit describes Assay for Transposase‐Accessible Chromatin with high‐throughput
sequencing (ATAC‐seq), a method for mapping chromatin accessibility genome‐wide. This
method probes DNA accessibility with hyperactive Tn5 transposase, which inserts
sequencing adapters into accessible regions of chromatin. Sequencing reads can then be
used to infer regions of increased accessibility, as well as to map regions of transcription‐
factor binding and nucleosome position. The method is a fast and sensitive alternative to …
Abstract
This unit describes Assay for Transposase‐Accessible Chromatin with high‐throughput sequencing (ATAC‐seq), a method for mapping chromatin accessibility genome‐wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription‐factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase‐seq for assaying chromatin accessibility genome‐wide, or to MNase‐seq for assaying nucleosome positions in accessible regions of the genome. © 2015 by John Wiley & Sons, Inc.
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