Site‐specific fluorescein labeling of human insulin
F Liu, WD Kohn, JP Mayer - Journal of Peptide Science, 2012 - Wiley Online Library
F Liu, WD Kohn, JP Mayer
Journal of Peptide Science, 2012•Wiley Online LibraryThree fluorescein derivatives of human insulin (HI, 1) labeled at positions NαA1, NαB1 and
NεB29 respectively, were synthesized using an N‐trifluoroacetyl‐based protecting group
scheme. The Tfa protecting group introduced by reaction with ethyl trifluoroacetate was
found to be stable in aqueous and organic media and efficiently removed under mild basic
conditions. Copyright© 2012 European Peptide Society and John Wiley & Sons, Ltd.
NεB29 respectively, were synthesized using an N‐trifluoroacetyl‐based protecting group
scheme. The Tfa protecting group introduced by reaction with ethyl trifluoroacetate was
found to be stable in aqueous and organic media and efficiently removed under mild basic
conditions. Copyright© 2012 European Peptide Society and John Wiley & Sons, Ltd.
Three fluorescein derivatives of human insulin (HI, 1) labeled at positions NαA1, NαB1 and NεB29 respectively, were synthesized using an N‐trifluoroacetyl‐based protecting group scheme. The Tfa protecting group introduced by reaction with ethyl trifluoroacetate was found to be stable in aqueous and organic media and efficiently removed under mild basic conditions. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.
Wiley Online Library