Brain ankyrin. A membrane-associated protein with binding sites for spectrin, tubulin, and the cytoplasmic domain of the erythrocyte anion channel.
JQ Davis, V Bennett - Journal of Biological Chemistry, 1984 - ASBMB
Brain ankyrin was purified from pig brain membranes in milligram quantities by a procedure
involving affinity chromatography on erythrocyte spectrinagarose. Brain ankyrin included two
polypeptides of Mr= 210,000 and 220,000 that were nearly identical by peptide mapping
and were monomers in solution. Brain ankyrin and erythrocyte ankyrin are closely related
proteins with the following properties in common: 1) shared antigenic sites, 2) high-affinity
binding to the spectrin beta subunit at the midregion of spectrin tetramers, 3) a binding site …
involving affinity chromatography on erythrocyte spectrinagarose. Brain ankyrin included two
polypeptides of Mr= 210,000 and 220,000 that were nearly identical by peptide mapping
and were monomers in solution. Brain ankyrin and erythrocyte ankyrin are closely related
proteins with the following properties in common: 1) shared antigenic sites, 2) high-affinity
binding to the spectrin beta subunit at the midregion of spectrin tetramers, 3) a binding site …