[HTML][HTML] Different T cell receptor affinity thresholds and CD8 coreceptor dependence govern cytotoxic T lymphocyte activation and tetramer binding properties
T cells have evolved a unique system of ligand recognition involving an antigen T cell
receptor (TCR) and a coreceptor that integrate stimuli provided by the engagement of
peptide-major histocompatibility complex (pMHC) antigens. Here, we use altered pMHC
class I (pMHCI) molecules with impaired CD8 binding (CD8-null) to quantify the contribution
of coreceptor extracellular binding to (i) the engagement of soluble tetrameric pMHCI
molecules,(ii) the kinetics of TCR/pMHCI interactions on live cytotoxic T lymphocytes (CTLs) …
receptor (TCR) and a coreceptor that integrate stimuli provided by the engagement of
peptide-major histocompatibility complex (pMHC) antigens. Here, we use altered pMHC
class I (pMHCI) molecules with impaired CD8 binding (CD8-null) to quantify the contribution
of coreceptor extracellular binding to (i) the engagement of soluble tetrameric pMHCI
molecules,(ii) the kinetics of TCR/pMHCI interactions on live cytotoxic T lymphocytes (CTLs) …