Induction of tissue factor expression on human umbilical vein endothelial cells by cell-specific HLA class I antibody: preliminary data

A Naji, F Deschaseaux, E Racadot, C Ferrand… - Transplantation …, 2005 - Elsevier
Donor-specific antibodies may play an important role in the development of chronic allograft
rejection process. However, the mechanisms leading to intimal vascular proliferation and
fibrosis remain poorly understood. The aim of this study was to examine whether donor-
specific HLA antibodies induce overexpression of tissue factor (TF) by endothelial cells. HLA
typed human umbilical vein endothelial cells (HUVEC) were incubated for 1 to 12 hours with
LPS (10 μg/mL), and increasing concentrations (1 to 500 μg/mL) of anti-HLA A1 antibody …