Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes

N Takahashi, T Yoshizaki, N Hiranaka, T Suzuki… - Biochemical and …, 2011 - Elsevier
N Takahashi, T Yoshizaki, N Hiranaka, T Suzuki, T Yui, M Akanuma, K Oka, K Kanazawa…
Biochemical and Biophysical Research Communications, 2011Elsevier
Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in
adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome.
However, the significance of this reduction remains unclear. This study investigated if and
how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels
of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by
introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and …
Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-κB activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.
Elsevier