[HTML][HTML] A primer for morpholino use in zebrafish
Zebrafish, 2022•liebertpub.com
Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used
in zebrafish (Danio rerio). This review discusses common practices for the design,
preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting
effects of morpholinos are discussed as well as method to minimize this potentially
confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of
morpholinos are summarized and contextualized with respect to the complementary, DNA …
in zebrafish (Danio rerio). This review discusses common practices for the design,
preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting
effects of morpholinos are discussed as well as method to minimize this potentially
confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of
morpholinos are summarized and contextualized with respect to the complementary, DNA …
Abstract
Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
Mary Ann Liebert