[HTML][HTML] Regulation of the erythroid Kruppel-like factor (EKLF) gene promoter by the erythroid transcription factor GATA-1.

M Crossley, AP Tsang, JJ Bieker, SH Orkin - Journal of Biological Chemistry, 1994 - Elsevier
M Crossley, AP Tsang, JJ Bieker, SH Orkin
Journal of Biological Chemistry, 1994Elsevier
Erythroid Kruppel-like factor (EKLF) is an erythroid-specific transcription factor that binds a
CACCC motif found in the human beta-globin gene promoter. We have studied the promoter
of the EKLF gene and identified binding sites for the transcription factors GATA-1 and
CCAAT-binding Protein 1 (CP1). We show that both types of binding sites are required for
full activity, and that the GATA motif at-60 is essential. The EKLF promoter can be directly
activated in nonerythroid cells in cotransfection experiments by forced expression of GATA …
Erythroid Kruppel-like factor (EKLF) is an erythroid-specific transcription factor that binds a CACCC motif found in the human beta-globin gene promoter. We have studied the promoter of the EKLF gene and identified binding sites for the transcription factors GATA-1 and CCAAT-binding Protein 1 (CP1). We show that both types of binding sites are required for full activity, and that the GATA motif at -60 is essential. The EKLF promoter can be directly activated in nonerythroid cells in cotransfection experiments by forced expression of GATA-1. These results suggest that EKLF is dependent on GATA-1 for its expression and lies downstream of, or coincident with, GATA-1 in a regulatory hierarchy in erythroid development.
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