Transgenic mice overexpressing the 5-hydroxytryptamine transporter gene in smooth muscle develop pulmonary hypertension

C Guignabert, M Izikki, LI Tu, Z Li, P Zadigue… - Circulation …, 2006 - Am Heart Assoc
C Guignabert, M Izikki, LI Tu, Z Li, P Zadigue, AM Barlier-Mur, N Hanoun, D Rodman…
Circulation research, 2006Am Heart Assoc
One intrinsic abnormality of pulmonary artery smooth muscle cells (PA-SMCs) in human
idiopathic pulmonary hypertension (iPH) is an exaggerated proliferative response to
internalized serotonin (5-HT) caused by increased expression of the 5-HT transporter (5-
HTT). To investigate whether 5-HTT overexpression in PA-SMCs is sufficient to produce PH,
we generated transgenic mice overexpressing 5-HTT under the control of the SM22
promoter. Studies in SM22-LacZ+ mice showed that the transgene was expressed …
One intrinsic abnormality of pulmonary artery smooth muscle cells (PA-SMCs) in human idiopathic pulmonary hypertension (iPH) is an exaggerated proliferative response to internalized serotonin (5-HT) caused by increased expression of the 5-HT transporter (5-HTT). To investigate whether 5-HTT overexpression in PA-SMCs is sufficient to produce PH, we generated transgenic mice overexpressing 5-HTT under the control of the SM22 promoter. Studies in SM22-LacZ+ mice showed that the transgene was expressed predominantly in SMCs of pulmonary and systemic vessels. Compared with wild-type mice, SM22–5-HTT+ mice exhibited a 3- to 4-fold increase in lung 5-HTT mRNA and protein, together with increased lung 5-HT uptake activity, but no changes in platelet 5-HTT activity or blood 5-HT levels. At 8 weeks of age, SM22–5-HTT+ mice exhibited PH, with marked increases in right ventricular systolic pressure (RVSP), right ventricle/left ventricle+septum ratio, and muscularization of distal pulmonary vessels, but no changes in systemic arterial pressure. PH worsened with age. Except a marked decrease in Kv channels, no changes in the lung expression of mediators of pulmonary vascular remodeling were observed in SM22–5-HTT+ mice. Compared with wild-type mice, SM22–5-HTT+ mice showed depressed hypoxic pulmonary vasoconstriction contrasting with greater severity of hypoxia- or monocrotaline-induced PH. These results show that increased 5-HTT expression in PA-SMCs, to a level close to that found in human iPH, lead to PH in mice. They further support a central role for 5-HTT in the pathogenesis of PH, making 5-HTT a potential therapeutic target.
Am Heart Assoc