Filaments in the microvillous border of intestinal cells
JD McNabb, E Sandborn - The Journal of cell biology, 1964 - ncbi.nlm.nih.gov
JD McNabb, E Sandborn
The Journal of cell biology, 1964•ncbi.nlm.nih.govMATERIALS AND METHODS Adult Sprague-Dawley rats were perfused through the left
ventricle first with balanced salt (10) to remove the blood. They were then fixed by perfusion
with a mixture of 6.5 per cent glutaraldehyde and 2 per cent acrolein in a phosphate buffer
(pH 7.5)(11). After 15 minutes, the duodenum was removed, fixed for an additional hour in
the same aldehyde mixture, and rinsed in Veronal-acetate buffer for 15 minutes. It was then
postfixed in Veronal-acetate-buffered 2 per cent osmium tetroxide (8) and embedded in …
ventricle first with balanced salt (10) to remove the blood. They were then fixed by perfusion
with a mixture of 6.5 per cent glutaraldehyde and 2 per cent acrolein in a phosphate buffer
(pH 7.5)(11). After 15 minutes, the duodenum was removed, fixed for an additional hour in
the same aldehyde mixture, and rinsed in Veronal-acetate buffer for 15 minutes. It was then
postfixed in Veronal-acetate-buffered 2 per cent osmium tetroxide (8) and embedded in …
MATERIALS AND METHODS
Adult Sprague-Dawley rats were perfused through the left ventricle first with balanced salt (10) to remove the blood. They were then fixed by perfusion with a mixture of 6.5 per cent glutaraldehyde and 2 per cent acrolein in a phosphate buffer (pH 7.5)(11). After 15 minutes, the duodenum was removed, fixed for an additional hour in the same aldehyde mixture, and rinsed in Veronal-acetate buffer for 15 minutes. It was then postfixed in Veronal-acetate-buffered 2 per cent osmium tetroxide (8) and embedded in Epon (6). Sections were stained with lead according to
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