SOX9 is required for the differentiation of paneth cells in the intestinal epithelium
Y Mori–Akiyama, M Van den Born, JH Van Es… - Gastroenterology, 2007 - Elsevier
Gastroenterology, 2007•Elsevier
Background & Aims: The transcription factor SOX9 has been shown previously to have an
essential role in the differentiation of a small number of discrete cell lineages. In the
intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt
signaling. Methods: To examine the function of SOX9 in the intestine, we inactivated the
Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9
gene and a Villin-Cre transgene. Results: In the absence of SOX9, Paneth cells were not …
essential role in the differentiation of a small number of discrete cell lineages. In the
intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt
signaling. Methods: To examine the function of SOX9 in the intestine, we inactivated the
Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9
gene and a Villin-Cre transgene. Results: In the absence of SOX9, Paneth cells were not …
Background & Aims
The transcription factor SOX9 has been shown previously to have an essential role in the differentiation of a small number of discrete cell lineages. In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling.
Methods
To examine the function of SOX9 in the intestine, we inactivated the Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9 gene and a Villin-Cre transgene.
Results
In the absence of SOX9, Paneth cells were not formed, but the differentiation of other intestinal epithelial cell types was unaffected. The lack of SOX9 also lead to crypt enlargement, to a marked increase in cell proliferation throughout the crypts, and to replacement of the Paneth cells by proliferating epithelial cells.
Conclusions
We conclude that SOX9 is required for the differentiation of Paneth cells. Our results elucidate an essential step in the differentiation of gut epithelium.
Elsevier