CD155 on human vascular endothelial cells attenuates the acquisition of effector functions in CD8 T cells

NK Escalante, A von Rossum, M Lee… - … , and vascular biology, 2011 - Am Heart Assoc
NK Escalante, A von Rossum, M Lee, JC Choy
Arteriosclerosis, thrombosis, and vascular biology, 2011Am Heart Assoc
Objective—CD155 is a cell surface protein that has recently been described to exert immune
regulatory functions. We have characterized the expression of CD155 on human vascular
endothelial cells (ECs) and examined its role in the regulation of T-cell activation. Methods
and Results—CD155 was expressed on resting human vascular ECs and was upregulated
in an interferon-γ (IFNγ)–dependent manner. When the function of CD155 in regulating T-
cell activation was examined, antibody-mediated neutralization of CD155 did not affect CD8 …
Objective
CD155 is a cell surface protein that has recently been described to exert immune regulatory functions. We have characterized the expression of CD155 on human vascular endothelial cells (ECs) and examined its role in the regulation of T-cell activation.
Methods and Results
CD155 was expressed on resting human vascular ECs and was upregulated in an interferon-γ (IFNγ)–dependent manner. When the function of CD155 in regulating T-cell activation was examined, antibody-mediated neutralization of CD155 did not affect CD8 T-cell proliferation in response to stimulation with ECs. However, neutralization of CD155 activity or small interfering RNA-mediated inhibition of CD155 expression in ECs increased expression of IFNγ and cytotoxic effector function in activated CD8 T cells.
Conclusion
CD155 is an IFNγ-inducible immune regulatory protein on the surface of human ECs that attenuates the acquisition of effector functions in CD8 T cells.
Am Heart Assoc