[PDF][PDF] Epidermal growth factor receptor in non–small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis

FR Hirsch, M Varella-Garcia, PA Bunn Jr… - Journal of clinical …, 2003 - researchgate.net
FR Hirsch, M Varella-Garcia, PA Bunn Jr, MV Di Maria, R Veve, RM Bremnes, AE Barón…
Journal of clinical oncology, 2003researchgate.net
Purpose: The epidermal growth factor receptor (EGFR) is frequently overexpressed in non–
small-cell lung carcinoma (NSCLC), and EGFR inhibitors are promising new therapeutic
agents. The molecular mechanisms responsible for EGFR overexpression are poorly
understood. Materials and Methods: Gene copy number and protein status of EGFR were
investigated in microarrayed tumors from 183 NSCLC patients, including squamous cell
carcinoma (SCC; 89 patients) and non-SCC (94 patients) histologies. Protein expression …
Purpose: The epidermal growth factor receptor (EGFR) is frequently overexpressed in non–small-cell lung carcinoma (NSCLC), and EGFR inhibitors are promising new therapeutic agents. The molecular mechanisms responsible for EGFR overexpression are poorly understood.
Materials and Methods: Gene copy number and protein status of EGFR were investigated in microarrayed tumors from 183 NSCLC patients, including squamous cell carcinoma (SCC; 89 patients) and non-SCC (94 patients) histologies. Protein expression was assessed by immunohistochemistry on a scale from 0 to 400 (percentage of positive cells staining intensity). Gene and chromosome 7 copy numbers were identified by fluorescent in situ hybridization (FISH).
Results: EGFR protein overexpression was observed in
62% of the NSCLC (25% scored 201 to 300; 37% scored 301 to 400), more frequently in SCC than non-SCC (82% v 44%;
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