RBPJ inhibition impairs the growth of lung cancer

Q Lv, R Shen, J Wang - Tumor Biology, 2015 - Springer
Q Lv, R Shen, J Wang
Tumor Biology, 2015Springer
The exact effects of the modulation of Notch signaling pathway on cell growth have been
shown to depend on tumor cell type. Recombination signal-binding protein Jκ (RBPJ) is a
key transcription factor downstream of receptor activation in Notch signaling pathway. Here,
we evaluated the effects of RBPJ inhibition on the growth of lung cancer cells. We found that
a short hairpin interfering RNA (shRNA) for RBPJ efficiently inhibited RBPJ expression in
lung cancer cells, resulting in a significant decrease in the cell growth. Further analyses …
Abstract
The exact effects of the modulation of Notch signaling pathway on cell growth have been shown to depend on tumor cell type. Recombination signal-binding protein Jκ (RBPJ) is a key transcription factor downstream of receptor activation in Notch signaling pathway. Here, we evaluated the effects of RBPJ inhibition on the growth of lung cancer cells. We found that a short hairpin interfering RNA (shRNA) for RBPJ efficiently inhibited RBPJ expression in lung cancer cells, resulting in a significant decrease in the cell growth. Further analyses showed that RBPJ inhibition altered the levels of its downstream targets, including p21, p27, CDK2, Hes1, Bcl-2, and SKP2, to prevent the cells from growing. Our data thus suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human lung cancer.
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