The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon
occurring in different intestinal diseases. Several strategies to mitigate IRI are in
experimental stages; unfortunately, no consensus has been reached about the most
appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in
mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model.
Mice were divided into treated (IPC, TAC, and IPC+ TAC) and untreated groups before …