Transcriptional analysis of the B cell germinal center reaction
Proceedings of the National Academy of Sciences, 2003•National Acad Sciences
The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is
targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that
occur in B cells during GC transit (naïve B cells→ centroblasts→ centrocytes→ memory B
cells) by gene expression profiling. Naïve B cells, characterized by the expression of cell
cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical
proliferation program lacking c-Myc expression, switching to a proapoptotic program, and …
targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that
occur in B cells during GC transit (naïve B cells→ centroblasts→ centrocytes→ memory B
cells) by gene expression profiling. Naïve B cells, characterized by the expression of cell
cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical
proliferation program lacking c-Myc expression, switching to a proapoptotic program, and …
The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in B cells during GC transit (naïve B cells → centroblasts → centrocytes → memory B cells) by gene expression profiling. Naïve B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, and down-regulating cytokine, chemokine, and adhesion receptors. The transition from GC to memory cells is characterized by a return to a phenotype similar to that of naïve cells except for an apoptotic program primed for both death and survival and for changes in the expression of cell surface receptors including IL-2 receptor β. These results provide insights into the dynamics of the GC reaction and represent the basis for the analysis of B cell malignancies.
National Acad Sciences