Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.
M Loetscher, B Gerber, P Loetscher, SA Jones… - The Journal of …, 1996 - rupress.org
M Loetscher, B Gerber, P Loetscher, SA Jones, L Piali, I Clark-Lewis, M Baggiolini, B Moser
The Journal of experimental medicine, 1996•rupress.orgA human receptor that is selective for the CXC chemokines IP10 and Mig was cloned and
characterized. The receptor cDNA has an open reading frame of 1104-bp encoding a
protein of 368 amino acids with a molecular mass of 40,659 dalton. The sequence includes
seven putative transmembrane segments characteristic of G-protein coupled receptors. It
shares 40.9 and 40.3% identical amino acids with the two IL-8 receptors, and 34.2-36.9%
identity with the five known CC chemokine receptors. The IP10/Mig receptor is highly …
characterized. The receptor cDNA has an open reading frame of 1104-bp encoding a
protein of 368 amino acids with a molecular mass of 40,659 dalton. The sequence includes
seven putative transmembrane segments characteristic of G-protein coupled receptors. It
shares 40.9 and 40.3% identical amino acids with the two IL-8 receptors, and 34.2-36.9%
identity with the five known CC chemokine receptors. The IP10/Mig receptor is highly …
A human receptor that is selective for the CXC chemokines IP10 and Mig was cloned and characterized. The receptor cDNA has an open reading frame of 1104-bp encoding a protein of 368 amino acids with a molecular mass of 40,659 dalton. The sequence includes seven putative transmembrane segments characteristic of G-protein coupled receptors. It shares 40.9 and 40.3% identical amino acids with the two IL-8 receptors, and 34.2-36.9% identity with the five known CC chemokine receptors. The IP10/Mig receptor is highly expressed in IL-2-activated T lymphocytes, but is not detectable in resting T lymphocytes. B lymphocytes, monocytes and granulocytes. It mediates Ca2+ mobilization and chemotaxis in response to IP10 and Mig, but does not recognize the CXC-chemokines IL-8, GRO alpha, NAP-2, GCP-2. ENA78, PF4, the CC-chemokines MCP-1, MCP-2, MCP-3, MCP-4, MIP-1 alpha, MIP-1 beta. RANTES, 1309, eotaxin, nor lymphotactin. The exclusive expression in activated T-lymphocytes is of high interest since the receptors for chemokines which have been shown so far to attract lymphocytes, e.g., MCP-1, MCP-2, MCP-3, MIP-1 alpha, MIP-1 beta, and RANTES, are also found in monocytes and granulocytes. The present observations suggest that the IP10/Mig receptor is involved in the selective recruitment of effector T cells.
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