Lipoxin (LX)A4 and Aspirin-triggered 15-epi-LXA4 Inhibit Tumor Necrosis Factor 1α–initiated Neutrophil Responses and Trafficking: Regulators of a Cytokine …

M Hachicha, M Pouliot, NA Petasis… - The Journal of …, 1999 - rupress.org
The Journal of experimental medicine, 1999rupress.org
The impact of lipoxin A4 (LXA4) and aspirin-triggered lipoxins (ATLs) was investigated in
tumor necrosis factor (TNF)-α–initiated neutrophil (polymorphonuclear leukocyte) responses
in vitro and in vivo using metabolically stable LX analogues. At concentrations as low as 1–
10 nM, the LXA4 and ATL analogues each inhibited TNF-α–stimulated superoxide anion
generation and IL-1β release by human polymorphonuclear leukocytes. These LXA4-ATL
actions were time and concentration dependent and proved selective for TNF-α, as these …
The impact of  lipoxin A4 (LXA4) and aspirin-triggered lipoxins (ATLs) was investigated in tumor necrosis factor (TNF)-α–initiated neutrophil (polymorphonuclear leukocyte) responses in vitro and in vivo using metabolically stable LX analogues. At concentrations as low as 1–10 nM, the LXA4 and ATL analogues each inhibited TNF-α–stimulated superoxide anion generation and IL-1β release by human polymorphonuclear leukocytes. These LXA4-ATL actions were time and concentration dependent and proved selective for TNF-α, as these responses were not altered with either GM-CSF– or zymosan-stimulated cells. TNF-α–induced IL-1β gene expression was also regulated by both anti-LXA4 receptor antibodies and LXA4-ATL analogues. In murine air pouches, 15R/S-methyl-LXA4 dramatically inhibited TNF-α–stimulated leukocyte trafficking, as well as the appearance of both macrophage inflammatory peptide 2 and IL-1β, while concomitantly stimulating IL-4 in pouch exudates. Together, these results indicate that both LXA4 and ATL regulate TNF-α–directed neutrophil actions in vitro and in vivo and stimulate IL-4 in exudates, playing a pivotal role in immune responses.
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