Vascular endothelial cells (ECs) have a finite lifespan when cultured in vitro and eventually
enter an irreversible growth arrest state called" cellular senescence." It has been shown that
sphingolipids may be involved in senescence; however, the molecular links involved are
poorly understood. In this study, we investigated the signaling and functions of sphingosine
1-phosphate (S1P), a serum-borne bioactive sphingolipid, in ECs of different in vitro ages.
We observed that S1P-regulated responses are significantly inhibited and the S1P 1-3 …