Ceramide induces expression of the senescence histochemical marker, β-galactosidase, in human fibroblasts
RE Mouton, ME Venable - Mechanisms of ageing and development, 2000 - Elsevier
We recently showed that ceramide is elevated in senescence and that when administered to
low-passage cells induces biochemical changes characteristic of senescence. The in situ
histochemical marker β-galactosidase (β-Gal) has provided an important tool in the study of
cellular senescence. We investigated the ability of ceramide to induce the expression of β-
Gal and correlated this with cell proliferation. We find that de-C6-ceramide, induces the
expression of acidic β-Gal in fetal lung-derived Wi-38 human diploid fibroblasts. Our results …
low-passage cells induces biochemical changes characteristic of senescence. The in situ
histochemical marker β-galactosidase (β-Gal) has provided an important tool in the study of
cellular senescence. We investigated the ability of ceramide to induce the expression of β-
Gal and correlated this with cell proliferation. We find that de-C6-ceramide, induces the
expression of acidic β-Gal in fetal lung-derived Wi-38 human diploid fibroblasts. Our results …