High lipid and ceramide concentrations are hallmarks of obese and/or insulin resistant
skeletal muscle, yet little is known about its role on cell cycle and senescence. The purpose
of this study was to examine the role of ceramide on muscle senescence, and whether
metformin limited this response. Methods Low passage, proliferating C2C12 myoblasts were
treated with a control, 50 μM C2-ceramide (8 h), and/or 2 mM metformin, then examined for
insulin sensitivity, cell senescence, cell proliferation, cell cycle, protein expression of cell …