Effects of maternal n-3 fatty acid supplementation on placental cytokines, pro-resolving lipid mediators and their precursors

JA Keelan, E Mas, N D'Vaz, JA Dunstan, S Li… - …, 2015 - rep.bioscientifica.com
JA Keelan, E Mas, N D'Vaz, JA Dunstan, S Li, AE Barden, PJ Mark, BJ Waddell, SL Prescott
Reproduction, 2015rep.bioscientifica.com
The aim of this study was to determine whether supplementation with fish oil-derived n-3
polyunsaturated fatty acids (n-3 PUFA) during pregnancy modifies placental PUFA
composition, the accumulation of specialised pro-resolving lipid mediators (SPMs,
specifically resolvins (Rv), protectins (PD) and upstream precursors) and inflammatory gene
expression. Placentas were collected from women (nZ51) enrolled in a randomised, placebo
controlled trial of n-3 PUFA supplementation from 20-week gestation. Lipids were extracted …
Abstract
The aim of this study was to determine whether supplementation with fish oil-derived n-3 polyunsaturated fatty acids (n-3 PUFA) during pregnancy modifies placental PUFA composition, the accumulation of specialised pro-resolving lipid mediators (SPMs, specifically resolvins (Rv), protectins (PD) and upstream precursors) and inflammatory gene expression. Placentas were collected from women (nZ51) enrolled in a randomised, placebo controlled trial of n-3 PUFA supplementation from 20-week gestation. Lipids were extracted for fatty acid analysis and SPMs were quantitated by mass spectrometry. Gene expression was determined by qRT-PCR. Using multiple regression analysis, data were correlated for placental n-3 PUFA and SPM levels with PUFA levels in maternal and cord blood erythrocytes. Supplementation with n-3 PUFAs increased placental docosahexaenoic acid (DHA) levels, but not eicosapentaenoic acid
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