Quantification and molecular analysis of plasmatic HLA‐BCw molecules with a locus B and Cw HLA specific mAb (B1. 23.2 mAb)
M Drouet, L Aussel, B Drenou… - International Journal of …, 1995 - Wiley Online Library
M Drouet, L Aussel, B Drenou, R Fauchet
International Journal of Immunogenetics, 1995•Wiley Online LibraryPlasmatic HLA class I molecules were analysed quantitatively and qualitatively with a B and
Cw locus‐specific mAb, B 1.23. 2. sHLA‐BCw were detected among all individuals with a
wide range of concentration. The HLA‐A29 (19) allele was significantly associated with an
increased sHLA‐BCw mean concentration. Three different isoforms of relative molecular
weights of 44, 39 and 36kDa were detected. The 39kDa class I heavy chain was not
detected after immunoprecipitation with mAb B 1.23. 2 in 17% of tested plasma, but was …
Cw locus‐specific mAb, B 1.23. 2. sHLA‐BCw were detected among all individuals with a
wide range of concentration. The HLA‐A29 (19) allele was significantly associated with an
increased sHLA‐BCw mean concentration. Three different isoforms of relative molecular
weights of 44, 39 and 36kDa were detected. The 39kDa class I heavy chain was not
detected after immunoprecipitation with mAb B 1.23. 2 in 17% of tested plasma, but was …
Summary
Plasmatic HLA class I molecules were analysed quantitatively and qualitatively with a B and Cw locus‐specific mAb, B 1.23.2. sHLA‐BCw were detected among all individuals with a wide range of concentration. The HLA‐A29(19) allele was significantly associated with an increased sHLA‐BCw mean concentration. Three different isoforms of relative molecular weights of 44, 39 and 36kDa were detected. The 39kDa class I heavy chain was not detected after immunoprecipitation with mAb B 1.23.2 in 17% of tested plasma, but was immunoprecipitated with W6/32 mAb.
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