Prostaglandin E2 modulates dendritic cell function via EP2 and EP4 receptor subtypes

H Harizi, C Grosset, N Gualde - Journal of Leucocyte Biology, 2003 - academic.oup.com
H Harizi, C Grosset, N Gualde
Journal of Leucocyte Biology, 2003academic.oup.com
We have reported previously that PGE2 inhibits dendritic cells (DC) functions. Because E
prostanoid receptor (EPR) subtypes involved in this action are unknown, expression and
functions of these receptors were examined in DC. Western blot and flow cytometry analyses
showed that all EPRs were coexpressed in DC. In a dose-dependent manner,
lipopolysaccharide (LPS) enhanced EP2R/EP4R but not EP1R/EP3R expressions. NS-398,
a cyclooxygenase (COX)-2-selective inhibitor, suppressed LPS-enhanced EP2R/EP4R …
Abstract
We have reported previously that PGE2 inhibits dendritic cells (DC) functions. Because E prostanoid receptor (EPR) subtypes involved in this action are unknown, expression and functions of these receptors were examined in DC. Western blot and flow cytometry analyses showed that all EPRs were coexpressed in DC. In a dose-dependent manner, lipopolysaccharide (LPS) enhanced EP2R/EP4R but not EP1R/EP3R expressions. NS-398, a cyclooxygenase (COX)-2-selective inhibitor, suppressed LPS-enhanced EP2R/EP4R expression, suggesting that COX-2-issued prostaglandin E2 (PGE2) modulates DC function through stimulation of specific EPR subtypes. Using selective agonists, we found that butaprost, an EP2R agonist, and PGE1 alcohol, an EP2R and EP2R/EP4R agonist, inhibited major histocompatibility complex class II expression and enhanced interleukin-10 production from DC. However, no effect was observed with sulprostone and 17-phenyl-ω-trinor-PGE2, selective agonists for EP1R and EP1R/EP3R, respectively. Treatment of DC with dibutyryl cyclic adenosine monophosphate (cAMP), an analog of cAMP, mimics PGE2-induced, inhibitory effects. Taken together, our data demonstrate that EP2R/EP4R are efficient for mediating PGE2-induced modulation of DC functions.
Oxford University Press